Journal: STAR Protocols
Article Title: Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae
doi: 10.1016/j.xpro.2022.101900
Figure Lengend Snippet: MatlabTrack GUI for single-molecule tracking The various modules are bordered in red and numbered according to their sequence. 1. Press this button for loading the .tiff file (a time-lapse single-molecule movie), 2. Filter the images for denoising using Bandpass filter, 3. Create ROIs against your area of interest, where you want to track the single molecules (such as nucleus, spindle, transcription site visualized by GFP channel). A prompt will appear upon clicking “Add ROI” to load the reference image. 4. Detect particles using intensity thresholding, 5. Track the particles frame-to-frame to generate trajectories, 6. Manually check the tracks, delete, or edit, if necessary, 7. Analyze the tracks. Save the results as .mat file from the “File” menu.
Article Snippet: MatlabTrack v6.0 (MATLAB) , Ball et al. , https://sourceforge.net/projects/single-molecule-tracking.
Techniques: Sequencing